Influencia de los neuropéptidos,bombesina y calcitonina, sobre el crecimiento de las líneas celulares prostáticas, PC-3, DU 145 y LNCaP / Influence of neuropetides, bombesin and calcitonin, on prostatic cell lines PC-3, DU-145 and LNCaP growth

Las células neuroendocrinas están presentes en el tejido prostático normal y tumoral. Los neuropéptidos secretados por estas células tienen funciones biológicas que todavía no han sido totalmente esclarecidas. La presencia de células neuroendocrinas en el carcinoma prostático se relaciona con la progresión del tumor y un peor pronóstico. Estudiamos la influencia de bombesina y calcitonina sobre la proliferación de líneas tumorales prostáticas humanas andrógeno-insensibles, PC-3 y DU-145, y andrógeno-sensible, LNCaP , para ello utilizamos el análisis colorimétrico XTT y recuentos celulares con un hematocitómetro. El crecimiento de la línea celular PC-3 y DU-145 es estimulado por la bombesina y calcitonina pero estos peptidos no ejercen efecto estimulador sobre la línea celular LNCaP. Esto indica que la bombesina y calcitonina pueden modular la proliferación sobre las líneas andrógeno-insensibles "in vitro" y podrían actuar, por tanto, como factores paracrinos estimuladores del crecimiento sobre las células cancerosas prostáticas que se vuelven resistentes al tratamiento hormonal (AU)

[Effect of the neuropeptides bombesin and calcitonin on the growth of prostate cell lines, PC-3, DU 145, and LNCaP]. / Influencia de los neuropéptidos, bombesina y calcitonina, sobre el crecimiento de las líneas celulares prostáticas, PC-3, DU 145 y LNCaP.

Neuroendocrine cells are present in normal and tumoral prostate tissue, the neuropeptides secreted by this cells have a biological functions that have not been fully elucidated. The presence of neuroendocrine cells in prostatic carcinoma have been shown to increase tumor progression. We characterized the in vitro proliferative influence of bombesin and calcitonin in androgen-insensitive, PC-3 and DU-145, and androgen-sensitive, LNCaP, cell lines of human prostate cancers. The influence of these neuropeptides on proliferation were assessed using the colorimetric XTT assay and by cells counts with a hemocytometer. The growth of PC-3 and DU-145 cell lines is stimulated by bombesin and calcitonin but exerted any stimulatory effect on the proliferation of the LNCaP cell line. This indicate that bombesin and calcitonin can modulate proliferation of androgen-insensitive human prostate cell lines "in vitro" and may be potential paracrine growth promoters in stablished androgen irresponsive human prostatic carcinoma cells.

[Histomorphometry study of the effect of an inhibitor of 5-alpha reductase on the coating of human prostate explants in tissue culture]. / Estudio histomorfométrico del efecto de un inhibidor de la 5-alfa-reductasa sobre el recubrimiento de explantes de próstata humana en cultivo de tejido.

Histomorphometric study of hyperplastic human prostate explants coating grown "in vitro" to evaluate the response to a 5-alpha-reductase inhibitor (finasteride). Explants were grown for four weeks in three groups, one in growth medium alone (RPM1-1640), one supplemented with testosterone and one supplemented with testosterone plus finasteride. Explants underwent histological studies, quantifying the surface and thickness of the coating epithelium. Study of the values obtained from the different samples show significant differences (p < 0.001) for the coated surface and its thickness between groups supplemented with testosterone and testosterone + finasteride, and so it can be inferred that this compound (finasteride) would blockade basal cells proliferation and their migration on the explant surface.

[Assessment of the effect of a 5-alpha-reductase inhibitor on cultured explants of human prostate]. / Valoración de la acción de un inhibidor de la 5-alpha-reductasa sobre cultivos de explantes de próstata humana.

For the present work, cultures of hyperplastic human prostate explants were performed to evaluate the "in vitro" response of a 5-alpha reductase inhibitor-finasteride. Explants were cultured over a nine-week period in RPMI-1640 media supplemented with Penicillin-Streptomycin 4% and Fetal Bovine Serum 10%. Cultures were divided into one control group, one with testosterone added (1.25 x 10(4) micrograms/ml) and one which received testosterone (1.25 x 10(-4) micrograms/ml) plus finasteride (0,0038 mg/ml). Control explants showed involutive changes throughout the experience, which occurred later in those treated with testosterone and earlier and more marked in the case of finasteride. The testosterone-treated group showed intense positivity for PSA, a marker which reveals specific tissular functions, which remained uniformed in the control group through the entire experiment, and decreased gradually in the finasteride group. In summary, according to our work, culture of organs would be a useful tool for an in vitro evaluation of the hyperplastic human prostate response to the action of the 5-alpha reductase inhibitor, finasteride.

[Effect of bombesin on cultured prostatic cells]. / Evaluación de la acción de la bombesina sobre células prostáticas en cultivo.

The detection of immunoreactivity to bombesin-like peptides in endocrine-paracrine cells from normal and pathological prostates suggest they have a regulatory role over this structure. With the purpose of explaining the action of these peptides on the prostate tissue an analysis is made of the bombesin effect on single-layer cultures of normal (rat ventral prostate) and tumoral (PC-3 cell line) prostate tissue. After performing morphological studies through phase contrast microscopy, as well as using cell counts and immunohistochemical techniques (Ki-67) to study the influence of bombesin on cell proliferation, this has been shown to stimulate prostate cells proliferation "in vitro". This response was revealed with greater intensity at bombesin concentrations of 0.5 nM/ml on the rat ventral prostate and bombesin 1 nM/ml on the PC-3 cell line. No morphological differences were observed between the controls and the bombesin-containing microwells in the rat ventral prostate, but some greater size cell elements with irregular shape were seen in the PC-3 cultures, as well as an increased number of mitotic division shapes in the wells filled up with bombesin.

[Hemangioma of the testis: a problem of clinical diagnosis]. / Hemangioma testicular: un problema de diagnóstico clínico.

Vascular tumors of the testis are uncommon. A review of the literature showed that only 10 such cases have been reported. Another case of cavernous hemangioma of the testicle is described. Its clinical features, different histological patterns, treatment and its biological significance are discussed.